by EMBO Rep (2019)e47495
in : Michael Cuccione Childhood Cancer Research Program
Source: EMBP Press
The prominent mitotic roles for kinases such as cyclin‐dependent kinases (CDKs), Aurora kinases, Polo‐like kinases (PLKs) and Nima‐related kinases (NEKs) have been well characterised 1-7. However, the role of Casein Kinase 1 alpha (CK1α) in mitosis, if any, remains poorly defined. CK1α belongs to the CK1 family of Ser/Thr protein kinases that are implicated in diverse roles in a whole plethora of cellular processes, from Wnt signalling to the regulation of circadian rhythms 8. CK1 isoforms can phosphorylate hundreds of proteins in vitro, with a preference for Ser/Thr residues that conform to either a D/E‐X‐X‐S*/T* or pS/pT‐X‐X‐S*/T* motif 9.
A recent mitotic phosphoproteomic study found that around half of the identified phosphorylation sites conformed to the predicted CK1‐consensus phosphorylation motifs 10, potentially implying a significant role for CK1 catalytic activity in mitotic protein phosphorylation. Yet, there is a lack of definitive evidence regarding whether and how any of the CK1 isoforms, or CK1α in particular, are involved in mitosis.